Testosterone, Total and Free

Testosterone is a key steroid hormone involved in energy levels, muscle mass, libido, mood, and overall hormonal balance. This test measures both total testosterone (the overall amount circulating in the blood) and free testosterone, the biologically active portion that is not bound to proteins and available for the body to use.

Testing both values provides a more complete assessment of hormone status and helps identify cases where total testosterone may appear normal but free testosterone is reduced.

Testosterone testing helps assess:

• Low libido or sexual dysfunction
• Fatigue or low energy
• Reduced muscle mass
• Hormonal balance in men and women
• Symptoms of low testosterone
• Monitoring hormone therapy

Reference Range (Adult – General Guidance)

Total Testosterone:
Men: ~300–1000 ng/dL
Women: ~15–70 ng/dL

Free Testosterone:
Men: ~5–25 ng/dL
Women: ~0.3–3.5 pg/mL

(Ranges may vary slightly by laboratory.)

What Your Results May Mean

• High: May be associated with hormone therapy, anabolic steroid use, PCOS, or adrenal disorders.
• Low: May suggest hypogonadism, pituitary dysfunction, or age-related hormonal decline.
• Within Range: Suggests testosterone levels are within expected limits at the time of testing.

Processed through Mayo Clinic Laboratories.